RBS

Part:BBa_J100467:Design

Designed by: Julia Pharr   Group: Campbell M Lab   (2018-11-08)


A new RBS in E. coli to code for mScarlet protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Watson-Crick and Non-Watson-Crick base pairing was predominantly considered in the development of the new RBS. Our group had to determine the most effective combination of both kinds of base pairing in order to ensure the new RBS had a high affinity for the ribosomal RNA.


Source

Our group designed the new RBS ourselves, largely base on the research in the paper written by Dr. Campbell because of the evidence there that suggested that Non-Watson-Crick base pairing was advantageous in creating a new RBS in E. coli.

References